Mr.
Nguyen Si Tuan
Head of Department of Clinical Microbiology - Thongnhat Dongnai General Hospital, Vietnam
Title of the talk: Virulent factors and pan-genome analysis of the clinical multidrug-resistant Acinetobacter baumannii strains in a Vietnam Hospital
Abstract
Acinetobacter baumannii, an emerging infectious agent in humans, is a major cause of hospital-acquired infections worldwide due to its impressive propensity to rapidly acquire resistance elements to a wide range of antimicrobial agents. We sought to explore the genomic properties, phylogenetic relationships, and comparative genomics of this pathogen. For this purpose, A. baumannii strain DMS06670 was isolated from a sputum specimen obtained from a male patient with hospital-acquired pneumonia. Assembly of whole-genome shotgun sequences of strain DMS06669 and DMS06670 yielded an estimated genome size of 4.2Mb and 3.8 Mb. In this manner, the identification of potential antibiotic resistance genes was conducted, and we predicted that the probability of A. baumannii DMS06669 (our strain in previous study) and DMS06670 as a human pathogen is 85.8% and 85.3%, with 632 and 622 pathogenic families, respectively. Additionally, the clusters of orthologous groups (COGs) analysis in protein sequence of A. baumannii strain DMS06669 and DMS06670 was compared with the other four genomes showed that the orthologous protein clusters responsible for multi-drug exist inside highly antimicrobial resistant strains. Phylogenetic analysis revealed that, based on the average nucleotide identity value, A. baumannii DMS06670 is a sister group to the LAC-4 and BJAB0715 strains of A. baumannii while A. baumannii strain DMS06669 is a sister group to strains ATCC_17978, D1279779, ZW85-1, ab031, and SDF. Lastly, comparative analysis of twenty-three available genomes of A. baumanii strains revealed a pan-genome consisting of 15,883 genes. Our findings provide insight into the molecular mechanisms leading to antibiotic resistance in A. baumannii.
Keywords: Acinetobacter baumannii, pan-genome analysis, antibacterial agents, comparative whole-genome analysis
Biography
Si-Tuan has started working at Thongnhat Dongnai General Hospital from 2010. He has been the Head of Department of Clinical Microbiology – Thongnhat Dongnai General Hospital since 2014. He graduated with the master program in Molecular Biology of Microbiology from University Sud XI. After that, he graduated with a specialized in medical microbiology from the University of Medicine in Ho Chi Minh City in 2011. From 2013 to now, he is a PhD student specializing in microbiology at the National University of Ho Chi Minh City. He is currently a fourth-year medical student at the School of Medicine, Tan Tao University. Si-Tuan has published 7 scientific papers in specialized journals in Viet Nam and 2 scientific papers in ISI journals about Acinetobacter baumannii. He is the specialist in multidrug-resistant Acinetobacter baumannii field.
My research interests are centered on the clinical microbiology and molecular biology. I am generally interested in the antimicrobial resistance, pathogenesis and infection, bioinformatics and microbiome, nosocomial infectous diseases. More recently, I have become increasingly interested in bacterial genome. In particular, I focus on the evolution of drug resistance genes, the function of disease proteins, and the new drug resistance genes.
Publications: ORCID GoogleScholar